A method for small scale genomic DNA isolation from chickpea (Cicer arietinum L.)suitable for molecular marker analysis

Joshi, N. and Rawat, A. and Subramanian, R.B. and Rao, K.S. (2010) A method for small scale genomic DNA isolation from chickpea (Cicer arietinum L.)suitable for molecular marker analysis. Indian Journal of Science and Technology, 3 (12). pp. 1214-1217.

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Abstract

We describe a DNA isolation procedure for chickpea (Cicer arietinum L.) which is rapid and less expensive without involving ultra centrifugation or column purification steps. DNA preparation obtained from the present study was essentially suitable for PCR analysis which is one of the key steps in crop improvement programme through marker development and genetic engineering techniques. The yield of DNA ranged from 0.595- 5.550 μg/ml and the purity ratio was between 1.025- 2.010 indicating minimum levels of contaminating metabolites. The present protocol offers as a reliable, and consistent DNA isolation method for chickpea that yields large amount of pure & intact DNA.

Item Type: Article
Uncontrolled Keywords: DNA isolation, Cicer arietinum, PCR analysis.
Author Affiliation: B. R. Doshi School of Biosciences, Sardar Patel Maidan, Vadtal Road, Post Box No. 39, Sardar Patel University, Vallabh Vidyanagar-388120, Gujarat, India neha_sjoshi@yahoo.co.in, subramanianrb@gmail.com
Subjects: Crop Improvement
Divisions: Chickpea
Depositing User: Mr Siva Shankar
Date Deposited: 05 Oct 2012 04:46
Last Modified: 05 Oct 2012 04:46
Official URL: http://www.indjst.org
URI: http://eprints.icrisat.ac.in/id/eprint/8261

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