Somatic embryogenesis in wild relatives of cotton ( Gossypium Spp.)

Rao, A.Q. and Hussain, S.S. and Shahzad, M.S. (2012) Somatic embryogenesis in wild relatives of cotton ( Gossypium Spp.). Journal of Zhejiang University - Science B , 7 (4). pp. 291-298.

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Abstract

Wild cotton species can contribute a valuable gene pool for agronomically desirable cultivated tetraploid cultivars. In order to exploit diploid cotton a regeneration system is required to achieve transformation based goals. The present studies aimed at optimizing the conditions for regeneration of local varieties as well as wild species of cotton. Different callus induction media were tested with varying concentrations of hormones in which sucrose was used as nutritional source. Different explants (hypocotyls, cotyledon, root) were used to check the regeneration of both local cotton plants and wild relatives using T & G medium, BAP medium, CIM medium, EMMS medium, and cell suspension medium. Different stages of embryogenicity such as early torpedo stage, late torpedo stage, heart stage, globular stage and cotyledonary stage were observed in wild relatives of cotton. The results of this study pave the way for establishing future transformation methods.

Item Type:	Article
Uncontrolled Keywords:	Cotton - Callus - Somatic embryogenic - Wild species - Cell suspension culture
Author Affiliation:	University of the Punjab Institution Centre of Excellence in Molecular Biology Lahore 53700 Pakistan Lahore 53700 Pakistan
Subjects:	Soil Science and Microbiology Plant Physiology and Biochemistry > Plant Physiology Plant Physiology and Biochemistry > Biochemistry
Divisions:	General
Depositing User:	Mr. SanatKumar Behera
Date Deposited:	04 Oct 2012 15:22
Last Modified:	04 Oct 2012 15:22
Official URL:	http://dx.doi.org/10.1631/jzus.2006.B0291
URI:	http://eprints.icrisat.ac.in/id/eprint/8258

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