Wu, J. and Zhang, X. and Nie, Y. and Jin, S. and Liang, S. (2012) Factors affecting somatic embryogenesis and plant regeneration from a range of recalcitrant genotypes of Chinese cottons (Gossypium hirsutum L.). In Vitro Cellular & Developmental Biology - Plant, 40 (4). pp. 371-75.
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Abstract
A new protocol has been developed for the highly efficient somatic embryogenesis and plant regeneration of 10 recalcitrant Chinese cotton cultivars. Calluses and embryogenic calluses were induced on MSB1 medium containing the optimal combination of indolebutyric acid (IBA; 2.46 μM) and kinetin (KT; 2.32 μM). Up to 86.7% of embryogenic calluses differentiated into globular somatic embryos 2 mo. after culture on MSB2 medium containing double KNO3 and free of growth regultors. Up to 38.3% of the somatic embryos were converted into complete plants in 8 wk on MSB3 medium with l-asparagine (Asn)/l-glutamine (Gln) (7.6/13.6 mM). The plants were successfully transferred to soil and grew to maturity. With the protocol described here, we have obtained hundreds of regenerating plantlets from 10 recalcitrant cultivars, which is important for the application of tissue culture to cotton breeding and biotechnology.
| Item Type: | Article |
|---|---|
| Additional Information: | The authors thank Prof. Keith Lindsey (University of Durham, UK) and Dr. Jihong Liu for their fruitful review. Support from the National High-tech Project was appreciated. Much assistance from the National Key Laboratory of Crop Genetic Improvement is acknowledged. |
| Uncontrolled Keywords: | words: cotton; recalcitrant genotypes; IBA; in vitro culture; somatic embryos. |
| Author Affiliation: | National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, China |
| Subjects: | Soil Science and Microbiology Crop Improvement Plant Physiology and Biochemistry > Plant Physiology Plant Physiology and Biochemistry > Biochemistry |
| Divisions: | General |
| Depositing User: | Mr. SanatKumar Behera |
| Date Deposited: | 04 Oct 2012 15:15 |
| Last Modified: | 04 Oct 2012 15:15 |
| Official URL: | http://dx.doi.org/10.1079/IVP2004535 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/8257 |
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