Degola, F. and Dall'Asta, C. and Restivo, M. (2012) Development of a simple and high-throughput method for detecting aflatoxins production in culture media. Letters in Applied Microbiology, 55 (1). pp. 82-89.
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Abstract
Fungal conidia (Aspergillus flavus) were inoculated into the wells of a microplate containing 200 μl of different formulations of coconut-derived liquid medium. Time-dependent production of aflatoxins in the culture media was evaluated by a procedure relying on the UV-induced fluorescence emission by the toxin, using a microplate reader. These data were validated by comparison with the outputs of a conventional HPLC-based procedure. Determinations of aflatoxin concentration, according to the fluorimetric procedure, were performed either by withdrawing samples from the plates or by direct ‘in situ’ readings, the latter method reinforcing the high-throughput feature of the procedure. Fluorescence enhancers (cyclodextrins) did not ameliorate the sensitivity of the procedure to low concentrations of the toxin into the medium. The efficacy of the procedure was also validated by testing the effect on toxin yield of adding an antioxidant agent (α-lipoic acid) to the medium.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | food safety, mycotoxins, rapid methods |
| Author Affiliation: | Department of Genetics Biology of Microorganisms Anthropology Evolution, University of Parma, Parma, Italy |
| Subjects: | Soil Science and Microbiology > Microbiology |
| Divisions: | General |
| Depositing User: | Mr Siva Shankar |
| Date Deposited: | 01 Oct 2012 05:41 |
| Last Modified: | 01 Oct 2012 05:41 |
| Official URL: | http://dx.doi.org/10.1111/j.1472-765X.2012.03264.x |
| URI: | http://eprints.icrisat.ac.in/id/eprint/8174 |
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