MacFarlane, I.J. and Lees, E.M. and Conn, E.E.
(1975)
The in vitro biosynthesis of dhurrin, the cyanogenic glycoside of Sorghum bicolor.
Journal of Biological Chemistry, 250 (12).
pp. 4708-4713.
Abstract
A microsomal fraction from seedlings of Sorghum bicolor (Linn) Moench has been shown to catalyze the conversion of L-tyrosine to p-hydroxymandelonitrile via p-hydroxyphenylacetaldoxime. This transformation is consistent with the general pathway for cyanogenic glycoside biosynthesis proposed on the basis of in vivo experiments. When the microsomal fraction was combined with a protein fraction from the soluble portion of the cell and uridine diphosphate glucose, it was possible to demonstrate the synthesis of the cyanogenic glycoside dhurrin from L-tyrosine
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