Engel, K. and Asch, F. and Becker, M. (2012) In vivo staining of reduced iron by 2,2′ bipyridine in rice exposed to iron toxicity. Journal of Plant Nutrition and Soil Science, 175 (4). pp. 548-552.
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Abstract
A protocol for a novel method to visualize Fe(II) in rice tissues is proposed. The method is based on the selective formation of a purple-red color complex of 2,2′ bipyridine and Fe(II). Rice genotypes were exposed to 18 mM Fe(II) in nutrient solution for 2 d. Root systems of intact plants were subsequently placed in 2,2′ bipyridine solutions. The formation of the [Fe(bipy)<$>_3^{2+}<$>] color complex was visualized using bifocal microscopy. The method may improve the selection of genotypes during breeding for Fe-toxicity resistance of rice.
| Item Type: | Article |
|---|---|
| Additional Information: | The study was supported by the German Foundation of Science (DFG) under grant BE2491/3-1. |
| Uncontrolled Keywords: | bifocal microscopy, DPD, leaf bronzing, Oryza sativa L. , selection |
| Author Affiliation: | Institute of Crop Science and Resource Conservation, University of Bonn, Germany |
| Subjects: | Soil Science and Microbiology > Soil Sciences |
| Divisions: | Other Crops |
| Depositing User: | Mr Siva Shankar |
| Date Deposited: | 18 Sep 2012 05:17 |
| Last Modified: | 18 Sep 2012 05:18 |
| Official URL: | http://dx.doi.org/10.1002/jpln.201200096 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/7964 |
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