Bravo-Almonacid, F. and Rudoy, V. and Welin, B. and et al, . (2012) Field testing, gene flow assessment and pre-commercial studies on transgenic Solanum tuberosum spp. tuberosum (cv. Spunta) selected for PVY resistance in Argentina. Transgenic Research, 21. pp. 967-982.
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Abstract
Solanum tuberosum ssp. tuberosum (cv. Spunta) was transformed with a chimeric transgene containing the Potato virus Y (PVY) coat protein (CP) sequence. Screening for PVY resistance under greenhouse conditions yielded over 100 independent candidate lines. Successive field testing of selected lines allowed the identification of two genetically stable PVY-resistant lines, SY230 and SY233, which were further evaluated in field trials at different potatoproducing regions in Argentina. In total, more than 2,000 individuals from each line were tested along a 6-year period. While no or negligible PVY infection was observed in the transgenic lines, infection rates of control plants were consistently high and reached levels of up to 70–80%. Parallel field studies were performed in virus-free environments to assess the agronomical performance of the selected lines. Tubers collected from these assays exhibited agronomical traits and biochemical compositions indistinguishable from those of the non-transformed Spunta cultivar. In addition, an interspecific out-crossing trial to determine the magnitude of possible natural gene flow between transgenic line SY233 and its wild relative Solanum chacoense was performed. This trial yielded negative results, suggesting an extremely low probability for such an event to occur.
| Item Type: | Article |
|---|---|
| Additional Information: | This work was funded by Consejo Nacional de Investigaciones Cientı´ficas y Te´cnicas (CONICET), and Agencia Nacional de Promocio´n Cientı´fica y Tecnolo´gica (ANPCyT), Argentina. |
| Uncontrolled Keywords: | Field evaluation, Potato, Spunta, PVY, Coat protein, Transgenic, Resistance, Gene flow |
| Author Affiliation: | Instituto de Investigaciones en Ingenierı´a Gene´tica y Biologı´a Molecular (CONICET), Vuelta de Obligado 2490 (C1428ADN), Buenos Aires, Argentina |
| Subjects: | Crop Improvement |
| Divisions: | Other Crops |
| Depositing User: | Mr Siva Shankar |
| Date Deposited: | 12 Sep 2012 04:49 |
| Last Modified: | 12 Sep 2012 04:50 |
| Official URL: | http://dx.doi.org/10.1007/s11248-011-9584-9 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/7837 |
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