An integrated genetic linkage map of cultivated peanut (Arachis hypogaea L.) constructed from two RIL populations

Qin, H. and Feng, S. and Chen, C. and et al, . (2012) An integrated genetic linkage map of cultivated peanut (Arachis hypogaea L.) constructed from two RIL populations. TAG Theoretical and Applied Genetics, 124 (4). pp. 653-664.

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Abstract

Construction and improvement of a genetic map for peanut (Arachis hypogaea L.) continues to be an important task in order to facilitate quantitative trait locus (QTL) analysis and the development of tools for marker-assisted breeding. The objective of this study was to develop a comparative integrated map from two cultivated × cultivated recombinant inbred line (RIL) mapping populations and to apply in mapping Tomato spotted wilt virus (TSWV) resistance trait in peanut. A total of 4,576 simple sequence repeat (SSR) markers from three sources: published SSR markers, newly developed SSR markers from expressed sequence tags (EST) and from bacterial artificial chromosome end-sequences were used for screening polymorphisms. Two cleaved amplified polymorphic sequence markers were also included to differentiate ahFAD2A alleles and ahFAD2B alleles. A total of 324 markers were anchored on this integrated map covering 1,352.1 cM with 21 linkage groups (LGs). Combining information from duplicated loci between LGs and comparing with published diploid maps, seven homoeologous groups were defined and 17 LGs (A1–A10, B1–B4, B7, B8, and B9) were aligned to corresponding A-subgenome or B-subgenome of diploid progenitors. One reciprocal translocation was confirmed in the tetraploid-cultivated peanut genome. Several chromosomal rearrangements were observed by comparing with published cultivated peanut maps. High consistence with cultivated peanut maps derived from different populations may support this integrated map as a reliable reference map for peanut whole genome sequencing assembling. Further two major QTLs for TSWV resistance were identified for each RILs, which illustrated the application of this map.

Item Type: Article
Additional Information: This research was partially supported by funds provided by the USDA Agricultural Research Service, the Georgia Agricultural Commodity Commission for Peanuts, Peanut Foundation and National Peanut Board.
Uncontrolled Keywords: Genetic Mapping, Peanut, RIL Mapping, SSR
Author Affiliation: Department of Plant Pathology, University of Georgia, Tifton, GA, USA
Subjects: Crop Improvement > Genetics/Genomics
Divisions: Groundnut
Depositing User: Mr Siva Shankar
Date Deposited: 04 Sep 2012 06:11
Last Modified: 04 Sep 2012 06:11
Official URL: http://dx.doi.org/10.1007/s00122-011-1737-y
URI: http://eprints.icrisat.ac.in/id/eprint/7688

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