Anatomical and biochemical aspects of interaction between roots of chickpea and Fusarium oxysporum f. sp. ciceris race 2

Joshi, N. S. and Rao, K. S. and Subramanian, R. B. (2012) Anatomical and biochemical aspects of interaction between roots of chickpea and Fusarium oxysporum f. sp. ciceris race 2. Archives of Phytopathology and Plant Protection. 17 p..

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Abstract

Roots of the susceptible ‘‘JG-62’’ and resistant ‘‘WR-315’’ chickpeas (Cicer arietinum L.) were inoculated with a conidial suspension of Fusarium oxysporum f. sp. ciceris. Anatomical and biochemical studies were carried out in a timecourse manner to elucidate the infection process and plant defence reactions. Scanning electron microscope images revealed fungal colonisation in the root hair region. Early occurrence of fungal biofilms associated with the infected ‘‘JG-62’’ root epidermis was also visualised. After 96 h of inoculation, a gradual accumulation of polysaccharide positive deposits was observed in the xylem vessels of the infected ‘‘JG-62’’ roots. Fungal mycelium was observed in the vessel lumen of infected ‘‘JG-62’’ after 22 days of inoculation. Due to fungal invasion during this period, some of the vessels also appeared collapsed in ‘‘JG-62’’, whereas vessels in ‘‘WR-315’’ remained intact. The host plant defence responses specifically linked to the susceptible interactions were the induction of ascorbate peroxidase, guaiacol peroxidase and superoxide dismutase in roots and shoots.

Item Type: Article
Additional Information: This research was financially supported by University Grants Commission, New Delhi.The authors are grateful to the Pulses Research Station, Junagadh, India, for providing chickpea seeds and to Dr V. Gupta for providing the fungal culture.
Uncontrolled Keywords: chickpea, Fusarium oxysporum f. sp. ciceris, fungal biofilms
Author Affiliation: B. R. Doshi School of Biosciences, Sardar Patel University, Vallabh Vidyanagar 388120, Gujarat, India
Subjects: Plant Protection
Divisions: Chickpea
Depositing User: Mr Siva Shankar
Date Deposited: 29 Aug 2012 11:14
Last Modified: 31 Aug 2012 06:19
Official URL: http://dx.doi.org/10.1080/03235408.2012.674709
URI: http://eprints.icrisat.ac.in/id/eprint/7594

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