Deesuth, O. and Laopaiboon, P. and Jaisil, P. and Laopaiboon, L. (2012) Optimization of Nitrogen and Metal Ions Supplementation for Very High Gravity Bioethanol Fermentation from Sweet Sorghum Juice Using an Orthogonal Array Design. Energies , 5 (9). pp. 3178-3197.
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Abstract
Optimization of four parameters, i.e., zinc (Zn2+), magnesium (Mg2+), manganese (Mn2+) and yeast extract for bioethanol production from sweet sorghum juice by Saccharomyces cerevisiae NP 01 under very high gravity (VHG, 270 g·L−1 of total sugar) conditions was performed using an L9 (34) orthogonal array design. The fermentation was carried out at 30 °C in 500-mL air-locked Erlenmeyer flasks at the agitation rate of 100 rpm and the initial yeast cell concentration in the juice was approximately 5 × 107 cells·mL−1. The results showed that the order of influence was yeast extract > Mn2+ > Zn2+ > Mg2+ and the optimum nutrient concentrations for the ethanol fermentation were Zn2+, 0.01; Mg2+, 0.05; Mn2+, 0.04; and yeast extract, 9 g·L−1. The verification experiments under the optimum condition clearly indicated that the metals and nitrogen supplementation improved ethanol production efficiency under the VHG fermentation conditions. The ethanol concentration (P), yield (Yp/s) and productivity (Qp) were 120.58 ± 0.26 g·L−1, 0.49 ± 0.01 and 2.51 ± 0.01 g·L−1·h−1, respectively, while in the control treatment (without nutrient supplement) P, Yp/s and Qp were only 93.45 ± 0.45 g·L−1, 0.49 ± 0.00 and 1.30 ± 0.01 g·L−1·h−1, respectively.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | bioethanol; trace elements; nitrogen source; orthogonal array design; Saccharomyces cerevisiae; sweet sorghum juice; very high gravity (VHG) fermentation |
| Author Affiliation: | Khon Kaen University, Khon Kaen 40002, Thailand |
| Subjects: | Plant Protection Soil Science and Microbiology Statistics and Experimentation |
| Divisions: | Sorghum |
| Depositing User: | Mr. SanatKumar Behera |
| Date Deposited: | 28 Aug 2012 08:49 |
| Last Modified: | 28 Aug 2012 08:50 |
| Official URL: | http://dx.doi.org/10.3390/en5093178 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/7553 |
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