Kou, X. and Gao, J. and Xue, Z. and Zhang, Z. and Wang, H. and Wang, X. (2012) Purification and identification of antioxidant peptides from chickpea (Cicer arietinum L.) albumin hydrolysates. LWT - Food Science and Technology. pp. 1-8.
![[img]](http://eprints.icrisat.ac.in/style/images/fileicons/application_pdf.png) |
PDF (In Press, Corrected Proof)
- Accepted Version
Restricted to ICRISAT researchers only |
Abstract
Albumin derived from chickpea was hydrolyzed sequentially using Alcalase and Flavorzyme proteases for production of antioxidant peptides. To identify antioxidant peptides, chickpea albumin hydrolysate (CAH) was fractionated using size exclusion chromatography (G-25) methods. Antioxidant and free radical-scavenging activities of peptides purified from the CAH were evaluated using reducing power assays with 1,1-diphenyl-2-pycrylhydrazyl (DPPH) and TEAC as well as with hydroxyl radical-scavenging assays. The results demonstrated that chickpea peptide (CPe)-III exhibited the highest antioxidant activity compared to the other hydrolysates. CPe-III was identified to be RQSHFANAQP (1155 Da) by LC–ESI–MS/MS.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Chickpea albumin hydrolysate; Antioxidant peptide; Amino acid sequence; Free radical scavenging; LC–ESI–MS/MS |
| Author Affiliation: | Tianjin Forestry and Fruit Tree Institute, Tianjin 300112, China,Tianjin University, Tianjin 300072, China |
| Subjects: | Plant Production |
| Divisions: | Chickpea |
| Depositing User: | Mr. SanatKumar Behera |
| Date Deposited: | 28 Aug 2012 08:38 |
| Last Modified: | 28 Aug 2012 08:39 |
| Official URL: | http://dx.doi.org/10.1016/j.lwt.2012.08.002 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/7551 |
Actions (login required)
 |
View Item |
