Bakht, Jehan and Bano, A. and Shafi, M. and Dominy, Peter (2013) Effect of abscisic acid applications on cold tolerance in chickpea (Cicer arietinum L.). European Journal of Agronomy, 44. pp. 10-21.
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Abstract
A series of field experiments were undertaken at three locations in Khyber PukhtunKhwa (KPK) Province, Pakistan to assess the effects of low temperatures and phytohormone applications on chickpea (Cicer arietinum L.) growth and yield. These trials showed that ABA application (10−4 M) to 40-day-old plants (before the first seasonal frost) offset low temperature-induced growth and yield depression at harvest (200-day-old plants) by up to 17%. These yield improvements were mainly due to an increase in the number of seeds pod−1. Growth room experiments were carried out under controlled environmental conditions to establish how foliar application of 10−4 M ABA to 40-day-old plants might improve seed production at harvest. The foliar application of 10−4 M ABA had no detectable effect on endogenous shoot or root ABA levels four-days after spraying or on biomass when plants were maintained in warm conditions. When exposed to night temperatures of −2 °C, however, the endogenous ABA levels increased dramatically in both control and ABA-treated plants, but this rise was more rapid after ABA application (p < 0.01); after 14 days, these plants had gained significantly more biomass than the unsprayed controls (p < 0.05). No evidence was found to suggest ABA affected the osmotic or water balance of plants, but parallel experiments have shown ABA reduced low temperature-induced cell damage. Analysis of the proteome of the shoot tissues of ABA treated and untreated plants by 2-Dimensional Gel Electrophoresis identified several proteins that are induced by low temperatures and/or by ABA application in chickpea and which may be involved in conferring cold tolerance. Attempts were made to establish the identity of these proteins using mass spectrometry but in all cases the results were ambiguous; a more complete protein data base for legumes is required before the function of these proteins can be inferred.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Chickpea; Cicer arietinum; ABA; Cold tolerance; Proteome |
| Author Affiliation: | KPK Agricultural University Peshawar, Pakistan, Quaid-E-Azam University Islamabad, Pakistan, University of Glasgow, Glasgow G12 8QQ, UK |
| Subjects: | Plant Protection Plant Physiology and Biochemistry > Biochemistry |
| Divisions: | Chickpea |
| Depositing User: | Mr. SanatKumar Behera |
| Date Deposited: | 28 Aug 2012 08:15 |
| Last Modified: | 28 Aug 2012 08:58 |
| Official URL: | http://dx.doi.org/10.1016/j.eja.2012.07.006 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/7549 |
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