Validation of chickpea-STMS markers and DNA fingerprinting in lentil (Lens culinaris subsp. culinaris) cultivars of India

Agrawal, P.K. and Katiyar, A.K.. (2008) Validation of chickpea-STMS markers and DNA fingerprinting in lentil (Lens culinaris subsp. culinaris) cultivars of India. Indian Journal of Genetics and Plant Breeding, 68 (2). pp. 149-156.

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Abstract

A set of 31 lentil genotypes having indigenous and exotic origin were screened using 42 STMS markers derived from chickpea genome. Out of the 42 STMS primers tested, all of them gave amplified products in lentil. Among them 36 (85.7%) primers were found to be polymorphic among the lentil genotypes. At genetic distance of less than 0.29, all the 31 genotypes could be grouped into five clusters where cluster IV contained 19 genotypes, including the exotic genotype Precoz. Many primers gave genotype-specific amplified products which in combination(s) could be used for DNA fingerprinting of lentil genotypes. A set of 16 STMS markers have been identified which could differentiate all the lentil cultivars of India and, along with the morphological data can be used for identification and confirmation of the purity of the lentil cultivars

Item Type: Article
Author Affiliation: Indian Institute of Pulses Research, Kanpur 208 024
Subjects: Crop Improvement > Genetics/Genomics
Crop Improvement > Plant Breeding
Divisions: Chickpea
Depositing User: Mr T L Gautham
Date Deposited: 28 Nov 2010 20:46
Last Modified: 29 Dec 2010 20:19
Official URL: http://www.indianjournals.com/ijor.aspx?target=ijo...
URI: http://eprints.icrisat.ac.in/id/eprint/751

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