Race Profiling and Molecular Diversity Analysis of Fusarium oxysporum f.sp. ciceris Causing Wilt in Chickpea

Dubey, S. C. and Priyanka, K. and Singh, V. and Singh, B. (2012) Race Profiling and Molecular Diversity Analysis of Fusarium oxysporum f.sp. ciceris Causing Wilt in Chickpea. Journal of Phytopathology. 12 p..

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Abstract

Seventy isolates of Fusarium oxysporum f.sp. ciceris (Foc) causing chickpea wilt representing 13 states and four crop cultivation zones of India were analysed for their virulence and genetic diversity. The isolates of the pathogen showed high variability in causing wilt incidence on a new set of differential cultivars of chickpea, namely C104, JG74, CPS1, BG212, WR315, KWR108, GPF2, DCP92-3, Chaffa and JG62. New differential cultivars for each race were identified, and based on differential responses, the isolates were characterized into eight races of the pathogen. The same set of isolates was used for molecular characterization with four different molecular markers, namely random amplified polymorphic DNA, universal rice primers, simple sequence repeats and intersimple sequence repeats. All the four sets of markers gave 100% polymorphism. Unweighted paired group method with arithmetic average analysis grouped the isolates into eight categories at genetic similarities ranging from 37 to 40%. The molecular groups partially corresponded to the states of origin/chickpea-growing region of the isolates as well as races of the pathogen characterized in this study. ...

Item Type: Article
Additional Information: Authors are thankful to ICAR, New Delhi, India, for financial support through outreach project.
Uncontrolled Keywords: races, differential cultivars, genetic diversity, universal rice primers, intersimple sequence repeats, simple sequence repeats, random amplified polymorphic DNA, Fusarium oxysporum f.sp. ciceris
Author Affiliation: Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi, 110012, India
Subjects: Crop Improvement
Divisions: Chickpea
Depositing User: Mr B Krishnamurthy
Date Deposited: 16 Aug 2012 08:58
Last Modified: 16 Aug 2012 08:58
Official URL: http://dx.doi.org/10.1111/j.1439-0434.2012.01954.x
URI: http://eprints.icrisat.ac.in/id/eprint/7394

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