Evaluation and biochemical characterization of a distinctive pyoverdin from a pseudomonas isolated from chickpea rhizosphere

Tank, N. and Rajendran, N. and Patel, B. and Saraf, M. (2012) Evaluation and biochemical characterization of a distinctive pyoverdin from a pseudomonas isolated from chickpea rhizosphere. Brazilian Journal of Microbiology, 43 (2). pp. 639-648.

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Abstract

Microbial siderophores confiscate the available ferric ions around the roots and trigger a reaction resulting in plant growth promotion. In our study, a high level of siderophore production was observed from a newly isolated Pseudomonas sp. from the rhizosphere of Chickpea plants. Under an iron depleted condition in Standard Succinic acid medium a 1000 µgmL-1 of siderophore production was achieved. Increasing the concentration of iron showed an inverse relationship between growth and siderophore production. Fourier Transform Infrared Spectroscopy (FTIR) analysis of the purified crystals, its UV spectral analysis and High Pressure Liquid Chromatography (HPLC) revealed the identity of the siderophore as similar to that of pyoverdin with distinctive characters. Electron spray ionization mass spectroscopy (ESIMS) shows presence of abundance of A1 ions (419 m/z) and branching of amino acids from B1-B5. This pyoverdin contains a cyclic tetra peptide but Serine and Arginine are missing. Based on our analysis and deviations from the reported structure of pyoverdin it is suggested that this pseudomonas produces distinctly characterized pyoverdin siderophore.

Item Type: Article
Uncontrolled Keywords: Fluorescense, hydroxamates, collision activation, pyoverdin, Pseudomonas
Author Affiliation: Department of Microbiology, University School of Sciences, Gujarat University of Sciences, Gujarat University, Ahmedabad 380 009, Gujarat, India, Microbiology, Department of Biology, MASC, Kentucky State University, Frankfort, Kentucky, 4060, US
Subjects: Statistics and Experimentation
Plant Physiology and Biochemistry > Biochemistry
Divisions: Chickpea
Depositing User: Mr. SanatKumar Behera
Date Deposited: 16 Aug 2012 04:51
Last Modified: 16 Aug 2012 05:02
Official URL: http://dx.doi.org/10.1590/S1517-83822012000200028
URI: http://eprints.icrisat.ac.in/id/eprint/7381

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