Calmodulin mediated activation of acetyl-CoA carboxylase during aflatoxin production by Aspergillus parasiticus

Rao, J. P. and Subramanyam, C. (2000) Calmodulin mediated activation of acetyl-CoA carboxylase during aflatoxin production by Aspergillus parasiticus. Letters in Applied Mirobiology, 30. pp. 277-281.

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Abstract

The relevance of Ca2+ -calmodulin-mediated processes in channelling acetate for aflatoxin formation was investigated by studying the influence of trifluoperazine (an anticalmodulin agent) on [14C]-acetate incorporation and activity of acetyl-CoA carboxylase in Aspergillus parasiticus NRRL 2999. Culturing the organism in presence of 0+14 mmol l ÿ1 tri¯uoperazine resulted in 55% decrease of [14C]- acetate incorporation into aflatoxin B1, along with an 80% decrease in acetyl-CoA carboxylase activity at periods corresponding to maximal a¯atoxin production. Concomitant decrement (35%) in the activity of glucose-6-phosphate dehydrogenase indicated decreased availability of reduction potential (NADPH) required for aflatoxin biosynthesis. The ability of calmodulin to activate and trifluoperazine to inhibit acetyl-CoA carboxylase activity in a dose-dependent manner was also noted under in vitro conditions. The combined results suggest calmodulin-mediated activation of acetyl-CoA carboxylase as an important event for

Item Type: Article
Author Affiliation: Dept of Biochemistry, Osmania University, Hyderabad
Subjects: Plant Protection
Divisions: General
Depositing User: Mr Siva Shankar
Date Deposited: 13 Aug 2012 12:01
Last Modified: 13 Aug 2012 12:01
URI: http://eprints.icrisat.ac.in/id/eprint/7337

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