Murty, U R (1971) A Standardized Cotton Blue Stain for Pollen Germination and Growth in Andropogoneae Grasses. Stain Technology, 46 (5). pp. 239-243.
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Abstract
Styles along with ovaries were dissected from spikelets of sorghum and other grasses and fixed in 1:3 acetic alcohol at 0.5 hr intervals for 3 hr after pollination. The material was hydrated and then macerated in 50% HCl for 10 min at 56 C, washed in running water for 30 min, cleared in lactophenol for 35 min at 60 C, stained in 1% cotton blue for 50 min at 50 C, and destained for 1 hr in lactophenol at room temperature. Styles were dissected from the ovary in a drop of lactophenol. Slight pressure on the coverslip with the blunt end of a needle and a little warming spreads the stylar tissue and reveals the darkly stained pollen tubes.
| Item Type: | Article |
|---|---|
| Author Affiliation: | Regional Research Station, Indian Agricultural Research Institute, Hyderabad, 30, India |
| Subjects: | Crop Improvement |
| Divisions: | Sorghum |
| Depositing User: | Siva Shankar |
| Date Deposited: | 16 Oct 2010 05:12 |
| Last Modified: | 20 Oct 2010 10:09 |
| Official URL: | http://dx.doi.org/10.3109/10520297109067864 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/73 |
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