Patil, S.M. and Sawardekar, S.V. and Bhave, S.G. and Sawant, S.S. and Jambhale, N.D. and Gokhale, N.B.
(2009)
Development of somaclones and their genetic diversity analysis through RAPD in Finger millet (Eleusine coracana L. Gaertn.).
Indian Journal of Genetics and Plant Breeding, 69 (2).
pp. 132-139.
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Abstract
While developing a protocol for induction of variability in
vitro in finger millet, significant differences for callus
induction among genotypes, explant types, culture
medium and their interactions were observed. The
genotypes, IVT 10 (52.25%), IPGSM 10 (27.90%) and IVT 4
(23.60%) showed significantly higher frequencies of callus
induction as compared to other genotypes. All the
genotypes and explants showed highest callus induction
in MS + 2 mgl-1 2,4-D. MS medium induced early callus
than B5 media. Nine out of 12 genotypes showed somatic
embryogenesis and morphogenesis. However, genotypes
IPGSM 18, GOA 8/1 and AVT 11 failed to regenerate. Among
explants, mature embryo axes showed highest (25.68%)
regeneration response in nine genotypes. Half strength
MS medium supplemented with 0.2 mgl-1 IBA + 0.1 mgl-1
BAP and 2 per cent sucrose showed significantly higher
number of roots (10.25) in 6.55 days. The rooted plants
planted in pots containing soil, sand and vermiculite in
equal proportion showed 60% survivability in six
genotypes. A total of 10 primers were used for
amplification of genomic DNA of the 6 parents and their
45 variants for RAPD analysis. Out of 1374 amplified
fragments 1268 (92.59 %) DNA fragments were
monomorphic while the remaining 106 (7.41%) were
polymorphic. The RAPD based dendrogram indicated
subgrouping of IVT 10 variants and data showed genetic
distance between the variants
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