Durley, R.C. and Simpson, G.M. (1982) Leaf analysis for abscisic, phaseic and 3-indolylacetic acids by high-performance liquid chromatography. Journal of Chromatography A, 236 (1). pp. 181-188.
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Abstract
High-performance liquid chromatography (HLPC) is described for the purification and analysis of abscisic acid (AbA), phaseic acid (PA) and 3-indolylacetic acid (IAA) extracted from Sorghum bicolor leaves. The method is rapid, suitable for automation and capable of accommodating large numbers of samples. Detection limits are less than 1 ng for each hormone. Recovery efficiency is 75% for AbA and PA and 64% for IAA. After initial extraction and partition, AbA, PA and IAA were purified together as ammonium salts (ion pairs) on polyvinylpyrrolidone. This was followed by further purification and separation of the three hormones on preparative C18 reversed-phase HPLC (5-μm spherical particles). Analysis of AbA and PA was by absorption phase silica HPLC (5-μm spherical particles) with detection by UV absorption. Analysis of IAA was by C18 reversed-phase HPLC (5-μm spherical particles), with fluorescence detection
| Item Type: | Article |
|---|---|
| Author Affiliation: | University of Saskatchewan, Crop Science Dept. Saskatoon, Sask S7N 0W0 Canada |
| Subjects: | Plant Protection |
| Divisions: | Sorghum Soyabean |
| Depositing User: | Ms K Syamalamba |
| Date Deposited: | 08 Aug 2012 06:15 |
| Last Modified: | 08 Aug 2012 06:16 |
| Official URL: | http://dx.doi.org/10.1016/S0021-9673(00)82511-X |
| URI: | http://eprints.icrisat.ac.in/id/eprint/7246 |
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