Lansden, J.A. (1986) Determination of cyclopiazonic acid in peanuts and corn by thin layer chromatography. Journal of the Association of Official Analytical Chemists , 69 (6). pp. 964-966.
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Abstract
A thin layer chromatographic system including densitometry has been developed for determining cyclopiazonic acid in peanuts and corn. Samples are extracted with methanol-chloroform (20 + 80); the extract is stripped of most interferences by partitioning with aqueous sodium bicarbonate followed by acidification and repartitioning with chloroform. After thin layer chromatography and derivatization with dimethylaminobenzaldehyde-HCl spray, the toxin is quantitated by reflection densitometry at 540 nm. The recovery of cyclopiazonic acid averages 90% for peanuts and 85% for corn. The absolute detection limit is 25 ng per spot which translates to a detection limit of 125 micrograms/kg for a 50 g sample.
| Item Type: | Article |
|---|---|
| Author Affiliation: | U.S. Department of Agriculture, Agricultural Research Service, National Peanut Research Laboratory |
| Subjects: | Statistics and Experimentation > Experimentation Crop Improvement Plant Physiology and Biochemistry > Biochemistry |
| Divisions: | Groundnut Other Crops |
| Depositing User: | Mr. SanatKumar Behera |
| Date Deposited: | 08 Aug 2012 05:15 |
| Last Modified: | 08 Aug 2012 05:19 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/7238 |
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