Hahnau, S. and Weiler, E W (1991) Determination of the Mycotoxin Cyclopiazonic Acid by Enzyme Immunoassay. Journal of Agricultural and Food Chemistry, 39 (10). pp. 1887-1891.
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Abstract
Antisera highly selective for the mycotoxin cyc1opiazonic acid (CPA) were raised in rabbits. A competitive enzyme-linked imro.unosorbent assay (ELISA) for CPA, with use of alkaline phosphatase as tracer enzyme, was developed. The assay measures from 30 pg to 2 ng of CPA and showed an interassay, between·day variability of 14 % and an intraassay variability of 3.7%. An .efficient selection technique was devised that allows detection of CPA, even at low levels. in fungal strains growing as agar surface cultures. No prec1eaning of the samples is required. The technique allows efficient monitoring for fungal isolates producing CPA.
| Item Type: | Article |
|---|---|
| Author Affiliation: | Lehntuhl fUr Pf1anzenphysiologie. Ruhr-Universitiit Bochum, Universitiitsstrasse ISO. D(W)4630 Bochum. Germany |
| Subjects: | Plant Protection Plant Physiology and Biochemistry > Biochemistry |
| Divisions: | General |
| Depositing User: | Mr. SanatKumar Behera |
| Date Deposited: | 08 Aug 2012 04:57 |
| Last Modified: | 08 Aug 2012 04:59 |
| Official URL: | http://dx.doi.org/10.1021/jf00010a041 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/7235 |
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