Aflatoxin Production via Cross-Feeding of Pathway Intermediates during Cofermentation of Aflatoxin Pathway-Blocked Aspergillus parasiticus Mutants

Cleveland, T.E. and Bhatnagar, D. and Brown, R. L. (1991) Aflatoxin Production via Cross-Feeding of Pathway Intermediates during Cofermentation of Aflatoxin Pathway-Blocked Aspergillus parasiticus Mutants. Applied and Environmental Microbiology, 57 (10). pp. 2907-2911.

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Abstract

Cofermentation ofAspergillus parasiticus strains (SRRC 163 and SRRC 2043) blocked at different steps in the aflatoxin B1 (AFB,) biosynthetic pathway in a synthetic liquid medium or on seeds (cottonseed, corn kernels, and peanuts) resulted in production of AFBI. Strain SRRC 2043 accumulated O-methylsterigmatocystin (OMST), a late precursor in AFB1 biosynthesis, whereas SRRC 163 accumulated averantin, an early precursor in the pathway. Strain SRRC 2043 secreted large amounts of OMST in culture relative to the amounts of several other pathway intermediates secreted into media (by other AFB1 pathway-blocked strains). AFB, production occurred even when colonies of SRRC 163 and SRRC 2043 strains (producing no detectable AFB1) were grown together on an agar medium while physically separated from each other by a filter membrane (0.22-,um pore size). In addition, when mycelia of strain SRRC 163 were added to culture filtrates (containing no mycelia but containing secreted OMST) of strain SRRC 2043, AFB1 production occurred.

Item Type: Article
Uncontrolled Keywords: Aflatoxin, Aspergillus parasiticus
Author Affiliation: Southern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, 1100 Robert E. Lee Blvd., New Orleans, Louisiana 70124
Subjects: Plant Protection > Pesticides
Soil Science and Microbiology > Microbiology
Divisions: UNSPECIFIED
Depositing User: Mr Siva Shankar
Date Deposited: 08 Aug 2012 04:47
Last Modified: 19 Nov 2012 07:33
Official URL: http://aem.asm.org/content/57/10/2907
URI: http://eprints.icrisat.ac.in/id/eprint/7231

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