A Phytoalexin and Aflatoxin Producing Peanut Seed Culture System

Basha, S. M. and Cole, R. J. and Pancholy, S. K. (1994) A Phytoalexin and Aflatoxin Producing Peanut Seed Culture System. Peanut Science, 21. pp. 130-134.

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Abstract

An in uitro seed culture system was estabIished to grow peanut seed of different maturities viz. white, yellow, orange, brown and black, using a modified Murashige and Skoog medium. Under this system peanut seed of yellow, orange, brown and black maturity categories grew to maturity as measured by increase in their size and germinability. In oitro cultured seeds produced significant amounts of phytoalexins and were contaminated with aflatoxins following their inoculation with Aspergillus spp. while the noninoculated sterile controls did not produce any phytoalexins. Exposure of seed cultures to water stress using various concentrations of mannitol(0 to 1 M) and polyethylene glycol 8000 (0-30% w/v) caused asignificant decrease in their phytoalexin producing ability, and enhanced fungal growth compared to the nonstressed controls.

Item Type: Article
Additional Information: This research was supported by a grant from the USDMCSRS, Washington, DC and is based upon work supported by the USDA-ARS, No. 58-6604-2-005.
Uncontrolled Keywords: Aflatoxins, Aspergillus flavus, in vitro culture, mannitol, peanut, phytoalexins, polyethylene glycol.
Author Affiliation: Di\ision of .4gric. Sciences, Florida .4&M Uni\.., Tallahassee. FL 32307
Subjects: Plant Protection
Divisions: Groundnut
Depositing User: Mr Siva Shankar
Date Deposited: 07 Aug 2012 10:04
Last Modified: 07 Aug 2012 10:07
Official URL: http://www.peanutscience.com/doi/pdf/10.3146/i0095...
URI: http://eprints.icrisat.ac.in/id/eprint/7218

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