Assessment of genetic diversity among selected groundnut germplasm. I: RAPD analysis.

Dwivedi, S.L. and Gurtu, S. and Chandra, S. and Yuejin, W. and Nigam, S.N. (2001) Assessment of genetic diversity among selected groundnut germplasm. I: RAPD analysis. Plant Breeding , 120 (4). pp. 345-349.

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Abstract

Assessment of genetic diversity in a crop species is prerequisite to its improvement. The use of germplasm with distinct DNA profiles will help to generate genetically diversified breeding populations. The aims of the present experiment were to study molecular diversity among selected groundnut accessions and identify those with distinct DNA profiles for mapping and genetic enhancement. Twenty-six accessions and eight primers of a 10-mer were selected for random amplified polymorphic DNA assay. The genetic similarity (Sij) ranged from 59.0% to 98.8%, with an average of 86.2%. Both multidimensional scaling and unweighted pair-group method with arithmetic averages (UPGMA) dendrograms revealed the existence of five distinct clusters. However, this classification could not be related to known biological information about the accessions falling into different clusters. Some accessions with diverse DNA profiles (ICG 1448, 7101 and 1471, and ICGV 99006 and 99014) were identified for mapping and genetic enhancement in groundnut

Item Type: Article
Author Affiliation: International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, PO, 502324 AP. India.
Subjects: Crop Improvement > Genetics/Genomics
Crop Improvement > Plant Breeding
Divisions: Groundnut
Depositing User: Mr B Krishnamurthy
Date Deposited: 16 Oct 2010 05:21
Last Modified: 16 Oct 2010 05:21
Official URL: http://dx.doi.org/10.1046/j.1439-0523.2001.00613.x...
URI: http://eprints.icrisat.ac.in/id/eprint/72

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