Watson, T.G. and Daiber, K.H. and Novellie, L. (1974) Extraction of a salt-insoluble alpha -glucosidase from Sorghum vulgare grain. Phytochemistry, 13 (6). pp. 901-904.
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Abstract
Sorghum grain α-glucosidase may be either insoluble in sodium chloride under alkaline conditions, or partially soluble, depending upon the sorghum variety. A good correlation was found between the degree of sodium chloride insolubility of the grain α-glucosidase and the degree of water-insolubility of the malt amylases. Peptone, in the presence of sodium chloride, was effective in liberating sodium chloride insoluble α-glucosidase from grain. Similarly, the water-insoluble amylases of malt were solubilized by peptone in water. Maximum liberation of grain ga-glucosidase was only achieved, however, by using a combination of 8 M urea, 0·1 M sulphite, 5% peptone and 1% Triton. It is suggested that the insolubility in both enzymes is caused by insoluble tannin-enzyme complexes
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Sorghum vulgare; millet; birdproof; Gramineae; malt; α-glucosidase; amylase; enzyme extraction |
| Author Affiliation: | National Chemical Research Laboratory, Council for Scientific and Industrial Research, P.O. Box 395, Pretoria, Republic of South Africa |
| Subjects: | Plant Physiology and Biochemistry > Plant Physiology Plant Physiology and Biochemistry > Biochemistry |
| Divisions: | Sorghum |
| Depositing User: | Ms K Syamalamba |
| Date Deposited: | 02 Aug 2012 04:44 |
| Last Modified: | 02 Aug 2012 04:45 |
| Official URL: | http://dx.doi.org/10.1016/S0031-9422(00)91420-1 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/7122 |
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