Identification of Molecular Markers for a Aphid Resistance Gene in Sorghum and Selective Efficiency Using These Markers

Chang, J. and Cui, J. and Xue, W. and Zhang, Q. (2012) Identification of Molecular Markers for a Aphid Resistance Gene in Sorghum and Selective Efficiency Using These Markers. Journal of Integrative Agriculture, 11 (7). pp. 1086-1092.

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Abstract

In this study, an F2 segregated population obtained by hybridization between the aphid-sensitive sorghum strain Qiansan and aphid-resistant cultivar Henong 16 was used to establish an aphid-resistant pool and an aphid-sensitive pool. 192 pairs of AFLP (amplified fragment length polymorphism) marker primers were screened in these pools using BSA (bulked segregant analysis). Three pairs of -EcoR I-CTG/Mse I-CCT, -EcoR I-CTG/Mse I-CAT, and -EcoR I-AGT/Mse I-CCC showed linkage with aphis resistance. -EcoR I-CTG/Mse I-CCT-475, -EcoR I-CTG/Mse I-CAT-390, and -EcoR I-AGT/Mse I-CCC-350 (E42/M52-350) were mapped within 6, 10, and 13 cM distances with the aphid-resistant gene by using Mapmaker 3.0 software. The bands amplified by -EcoR I-CTG/Mse I-CCT-475 and -EcoR I-CTG/Mse I-CAT-390 were extracted, cloned, and sequenced. Specific primers of SCAR (sequence characterized amplified regions) were then designed from these bands. A specific band of 300 bp was amplified by a pair of SCAR primers designed based on the sequence obtained from the -EcoR I-CTG/Mse I-CAT-390 marker. The SCAR marker was named SCA50. The marker was used to detect the F2, BC1, and F2:3 populations. The selective efficiency was 86.8, 91.1, and 86.3% in the BC1, F2, and F2:3 populations, respectively. The average selective efficiency was 88.2%.

Item Type: Article
Uncontrolled Keywords: sorghum bicolor; aphid resistance gene; molecular marker; SCAR; molecular assistant selection
Author Affiliation: China Agricultural University, Beijing, Agricultural University of Hebei,Baoding , China Baoding Vocational and Technical College, Baoding
Subjects: Crop Improvement
Divisions: Sorghum
Depositing User: Mr. SanatKumar Behera
Date Deposited: 27 Jul 2012 03:05
Last Modified: 27 Jul 2012 03:06
Official URL: http://dx.doi.org/10.1016/S2095-3119(12)60101-4
URI: http://eprints.icrisat.ac.in/id/eprint/7014

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