Abdul-Hussain, S. and Varriano-Marston, E. (1982) Amylolysis of Pearl Millet Starch and Its Fractions by Pearl Millet Alpha-Amylase. Cereal Chemistry, 59 (5). pp. 351-355.
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Abstract
Alpha-amylase was isolated from germinated pearl millet and purified by glycogen-amylase complex formation. Germination resulted in a 120-fold increase in specific activity of the enzyme over that of alpha- amylase from mature grain. Amylolysis of millet starch and its fractions by purified alpha-amylase was studied by gel filtration. Raw millet starch was resistant to attack by alpha-amylase from germinated millet. Only limited degradation (26%) occurred after starch was incubated with the enzyme for 24 hr at 35 C. Millet amylose was readily hydrolyzed by purified millet alpha-amylase. During a 10-min reaction period at 35 C, 36% of the molecule was degraded to molecular weights of 40,000-10,000. Conversely, some portions of millet amylopectin were hydrolyzed slowly by alpha-amylase
| Item Type: | Article |
|---|---|
| Author Affiliation: | Department of Grain Science and Industry, Kansas State University, Manhattan 66506 |
| Subjects: | Plant Protection |
| Divisions: | Millet |
| Depositing User: | Mr B Krishnamurthy |
| Date Deposited: | 22 Jul 2012 13:50 |
| Last Modified: | 22 Jul 2012 13:50 |
| Official URL: | http://www.aaccnet.org/publications/cc/backissues/... |
| URI: | http://eprints.icrisat.ac.in/id/eprint/6890 |
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