Kaur, G. and Thokala, M. and Robert-Seilaniantz, A. and et al, . (2012) Subcellular targeting of an evolutionarily conserved plant defensin MtDef4.2 determines the outcome of plant–pathogen interaction in transgenic Arabidopsis. Molecular Plant Pathology. 15 p..
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Abstract
The Medicago truncatula gene encoding an evolutionarily conserved antifungal defensin MtDef4.2 was cloned and characterized. In silico expression analysis indicated that MtDef4.2 is expressed in many tissues during the normal growth and development of M. truncatula. MtDef4.2 exhibits potent broadspectrum antifungal activity against various Fusarium spp. Transgenic Arabidopsis thaliana lines in which MtDef4.2 was targeted to three different subcellular compartments were generated. These lines were tested for resistance to the obligate biotrophic oomycete Hyaloperonospora arabidopsidis Noco2 and the hemibiotrophic fungal pathogen Fusarium graminearum PH-1. MtDef4.2 directed to the extracellular space, but not to the vacuole or retained in the endoplasmic reticulum, conferred robust resistance to H. arabidopsidis. Siliques of transgenic Arabidopsis lines expressing either extracellularly or intracellularly targeted MtDef4.2 displayed low levels of resistance to F. graminearum, but accumulated substantially reduced levels of the mycotoxin deoxynivalenol....
| Item Type: | Article |
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| Additional Information: | We thank Ms Anita Snyder and Ms Lauren Pitt for help in the cloning of MtDef4.2, Drs Christopher Taylor (Ohio State University,Wooster, OH, USA) for providing Agrobacterium tumefaciens binary expression vector AKK1472, Uma Shankar Sagaram for technical discussions and Noah Fahlgren for data analysis. Thanks are due to the Fungal Genetics Stock Center (University of Missouri, Kansas City, MO, USA) for providing F. pseudograminearum 9095 and to Dr Don White (University of Illinois, Urbana Champagne, IL, USA) for providing F. proliferatum and F. verticillioides strains. Transgenic A. thaliana lines were generated at the Donald Danforth Plant Science Center (DDPSC) by Kevin Lutke. This work was supported by the National Science Foundation Award 0344444 to DS. |
| Author Affiliation: | Donald Danforth Plant Science Center, 975 North Warson Road, St. Louis, MO 63132, USA |
| Subjects: | Crop Improvement |
| Divisions: | General |
| Depositing User: | Mr Siva Shankar |
| Date Deposited: | 18 Jul 2012 03:03 |
| Last Modified: | 18 Jul 2012 03:04 |
| Official URL: | http://dx.doi.org/10.1111/J.1364-3703.2012.00813.X |
| URI: | http://eprints.icrisat.ac.in/id/eprint/6829 |
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