Characterization of an embryogenic cell suspension culture derived from cultured inflorescences of Pennisetum americanum (Pearl millet, Gramineae)

Vasil, V. and Vasil, I.K. (1982) Characterization of an embryogenic cell suspension culture derived from cultured inflorescences of Pennisetum americanum (Pearl millet, Gramineae). American Journal of Botany, 69 (9). pp. 1441-1449.

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Abstract

Suspensions composed of small, richly cytoplasmic starch-containing cells (embryogenic cells) and elongated thick-walled cells with large vacuoles were established from cultured inflorescence segments of pearl millet in Murashige and Skoog's medium supplemented with 2.5 mg 2,4-D/l + 5% coconut milk. When 2- to 3-wk-old suspensions were transferred to agar with reduced 2,4-D levels, hundreds of globular and early cotyledonary embryoids were produced which developed into green plants when transferred to medium + ABA. Atypical embryoids, some containing many shoot meristems and a leafy scutellum were also observed

Item Type: Article
Author Affiliation: Dep. of Bot., Florida Univ., Gainesville, FL 32611, USA.
Subjects: Crop Improvement
Divisions: Millet
Depositing User: Mr B Krishnamurthy
Date Deposited: 16 Jul 2012 14:30
Last Modified: 16 Jul 2012 14:30
Official URL: http://dx.doi.org/10.2307/2443105
URI: http://eprints.icrisat.ac.in/id/eprint/6787

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