Allele-specific amplification for the detection of ascochyta blight resistance in chickpea

Madrid, E. and Chen, W. and Rajesh, P. N. and et al, . (2012) Allele-specific amplification for the detection of ascochyta blight resistance in chickpea. Euphytica. 8 p..

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Abstract

A new co-dominant molecular marker, CaETR, was developed based on allelic sequence length polymorphism in an ethylene receptor-like gene located in the genomic region of a QTL (QTLAR1) conferring ascochyta blight resistance in chickpea. This marker not only discriminated resistance and susceptible phenotypes of chickpea to ascochyta blight, but also easily detected heterozygous genotypes. Using the CaETR marker in combination with a previously developed co-dominant SCAR marker (SCY17590) linked to another QTL (QTLAR2) it was possible to detect resistance alleles in 90 % of resistant accessions in a collection of landraces, advances breeding lines and cultivars, and also detected susceptible alleles in all cases.

Item Type: Article
Additional Information: This research was partly developed at the University of Cordoba and Instituto de Investigacio´n y Formacio´n Agraria y Pesca (IFAPA) Co´rdoba, Spain, belonging to the Campus de Excelencia Internacional Agroalimentario (ceiA3), and was supported by the Spanish Ministerio de Ciencia e Innovacio´n (MICINN; projects RTA2010-00059 and AGL2005- 07497-CO2-01/AGR.2003), co-financed by Fondo Europeo de Desarrollo Regional (FEDER).
Uncontrolled Keywords: Chickpea, Ascochyta blight, Allelespecific associated primers, Marker assisted selection
Author Affiliation: Department of Genetic, Co´rdoba University, Campus Rabanales, Edif. C5, 14071 Co´rdoba, Spain
Subjects: Plant Protection
Divisions: Chickpea
Depositing User: Mr Siva Shankar
Date Deposited: 16 Jul 2012 03:25
Last Modified: 16 Jul 2012 03:26
Official URL: http://dx.doi.org/10.1007/s10681-012-0753-6
URI: http://eprints.icrisat.ac.in/id/eprint/6761

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