Okamoto, M. and Hanada, A. and Kamiya, Y. and et al, . (2009) Measurement of Abscisic Acid and Gibberellins by Gas Chromatography/Mass Spectrometry. In: Plant Hormones: Methods and Protocols. Methods in Molecular Biology, 495 . Humana Press, pp. 53-60. ISBN 978-1-934115-32-9 (Print) 978-1-59745-477-3 (Online)
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Abstract
Gas chromatography–mass spectrometry (GC-MS)-based analysis is an accurate and sensitive method to quantify plant hormones. This method is commonly used for analysis of low-molecular-weight compounds, such as abscisic acid (ABA), gibberellins (GAs), auxins, and brassinosteroids. Procedures are composed of four major steps: extraction, fractionation, derivatization, and detection. Here, we describe a protocol for quantification of ABA and GAs.
| Item Type: | Book Section |
|---|---|
| Uncontrolled Keywords: | Abscisic acid, gibberellins, gas chromatography–mass spectrometry (GC-MS), internal standard, derivatization. |
| Author Affiliation: | Growth Regulation Research Group, RIKEN Plant Science Center, Tsurumi, Yokohama, Japan |
| Subjects: | Crop Improvement |
| Divisions: | General |
| Depositing User: | Mr Siva Shankar |
| Date Deposited: | 13 Jul 2012 03:33 |
| Last Modified: | 13 Jul 2012 03:48 |
| Official URL: | http://dx.doi.org/10.1007/978-1-59745-477-3_5 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/6721 |
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