Meristem culture of sweet potato (Ipomoea batatas)

Zamora, A.B. and Gruezo, S.Sm. (1993) Meristem culture of sweet potato (Ipomoea batatas). nt Genetic Resources Newsletter (91-92). pp. 25-28.

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Abstract

Experiments on culturing isolated meristems of sweet potato (Ipomoea hatatas) were conducted on four local cultivars to overcome a high :incidence of contam:ination and callus:ing of explants. Variables exam:ined :included explant size, cultivar, location of the meristem along the v:ine axis and culture media The aseptic culture of meristems was linproved by: (a) us:ing meristems derived from the tip to the fifth node of v:ines grown :in the greenhouse, not:ing the degree of pubescence of the cultivar to determ:ine the size of meristem to culture; and (b) the use of Murashige and Skoog's nutrient fonnulation + 1 ppm GA3 + 0.1 ppm Ki (WPM medium) or MS + too ml/l coconut water (MSC medium) as culture medium. Growth of isolated meristems was faster on WPM medium but MSC medium can be an alternative when purified growth regulators are not available.

Item Type: Article
Author Affiliation: Cellular Molecular Plant Biology Program, Institute of Plant Breeding, University of the Philippines College of Agriculture, Los Bãnos, Laguna 4031, Philippines.
Subjects: Plant Production
Plant Physiology and Biochemistry > Plant Physiology
Divisions: Other Crops
Depositing User: Mr. SanatKumar Behera
Date Deposited: 12 Jul 2012 05:14
Last Modified: 12 Jul 2012 05:14
URI: http://eprints.icrisat.ac.in/id/eprint/6705

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