Kovimandon, B. and Khan, Z.H. (2011) Seed Protein Profile as Substitute for Sorghum Cultivar Identification by Grow Our Test (GOT). Bioscience Discovery, 2 (2). pp. 151-154.
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Abstract
Conventionally Grow out Test (GOT) is used to assay the purity of sorghum hybrid seed lots on a representative sample of the seeds. ln GOT, plants are grown up to maturity and several morphological and floral characteristics are assessed to distinguish the hybrid. The hybrid seed produced is not immediately distributed to the farmers for raising the crop but GOT is performed in succeeding season to check the purity of hybrid seeds. This entails a lot of cost in terms of locked-up capitals and problems of storage. With the objective of replacing the GOT with biochemical (protein) assays, A-line (cytoplasmic male sterile), B-line (maintainer), R-line (restorer) and H-line (hybrid) have been screened by means of protein markers for polymorphisms. ln addition to this, eight open pollinated (pure line)varieties have also been screened by means of protein polymorphisms. A simple electrophoretic procedure for detecting purity of hybrids, their respective parents and varieties has been standardized. Seeds of the selected hybrids and their parents and varieties of sorghum (Sorghum bicolor (1.) Moench) have been used for assays. Electrophoretic protein profiles could be efficiently used for distinguishing varieties, hybrids and its parents and could be used as substitute of GOT.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Electrophoresis, Grow Out Test, Protein profile, Sorghum, Zymogram |
| Author Affiliation: | Department of Biochemistry, Shri Shivaji College of Arts, Commerce and Science, Near Railway station, Akola |
| Subjects: | Plant Production Statistics and Experimentation Crop Improvement |
| Divisions: | Sorghum |
| Depositing User: | Mr. SanatKumar Behera |
| Date Deposited: | 07 Jul 2012 07:22 |
| Last Modified: | 07 Jul 2012 07:23 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/6617 |
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