Environmental Factors Affecting the Adhesion of Probiotics to Rice Fiber Fractions

Fernando, W. M. A. D. B. and Flint, S. and Brennan, C. S. and et al, . (2011) Environmental Factors Affecting the Adhesion of Probiotics to Rice Fiber Fractions. Food Digestion, 2. pp. 26-36.

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Abstract

The adhesion of pure cultures of probiotics to insoluble dietary fiber (IDF1 and IDF2), soluble dietary fiber (SDF1 and SDF2), and total dietary fiber (TDF1 and TDF2) of two rice varieties (RR1 and RR2) was investigated. Bifidobacteria and Lactobacilli adhered to dietary fiber fractions within 15 to 30 min. A higher percentage of Bifidobacteria (40–70%) adhered to rice fiber than lactobacilli (<40%). Adhesion was not affected by NaCl, or Tween 80 but, was reduced by various sugars and polysaccharides (maltose, moltodextrin, amylopectin, and amylose). The optimal temperature for adhesion was 37 °C and live cells are important for adhesion. Adhesion was inhibited by protease and by low pH (pH 3–4). Adhesion was not influenced by proteins or peptides linked with the fiber but involved bacterial cell surface proteins or glycoproteins.

Item Type:	Article
Additional Information:	Thanks to Associate Professor Hugh Morton, Massey University, for his advice on the statistical analyses used in this study and to Professor Roger Lentil, Massey University, for proofreading this manuscript.
Uncontrolled Keywords:	Dietary fiber, Adhesion, Environmental factors, Pure cultures
Author Affiliation:	Institute of Food Nutrition and Human Health, Massey University, Private Bag 11222, Palmerston North, New Zealand
Subjects:	Plant Protection Environmental Science > Environment
Divisions:	Other Crops
Depositing User:	Mr Siva Shankar
Date Deposited:	04 Jul 2012 03:37
Last Modified:	04 Jul 2012 03:38
Official URL:	http://dx.doi.org/10.1007/s13228-011-0012-7
URI:	http://eprints.icrisat.ac.in/id/eprint/6512

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