Isolation of AhDHNs from Arachis hypogaea L. and evaluation of AhDHNs expression under exogenous abscisic acid (ABA) and water stress

Su, L. and Xu, L. and Chen, Y. and Li, L. (2012) Isolation of AhDHNs from Arachis hypogaea L. and evaluation of AhDHNs expression under exogenous abscisic acid (ABA) and water stress. African Journal of Biotechnology, 11 (51). pp. 11221-11229.

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Abstract

The peanut (Arachis hypogaea L.) is an important oil and cash crop all over the world. It is mostly planted in arid and semi-arid regions. To determine the mechanism by which dehydrins (DHNs) are regulated by abscisic acid (ABA) in peanuts, three Arachis hypogaea L. dehydrins (AhDHNs) were isolated from peanut plants and sequenced. By blasting the protein sequences of these AhDHNs, AhDHN1 was found belonging to the YnSKn subfamily. AhDHN2 and AhDHN3 were found belonging to the SKn and YnKn types, respectively. 100 μM ABA enhanced AhDHNs expression in peanut leaves. When peanut plants were treated with ABA and then with the ABA synthesis inhibitor sodium tungstate 12 h later, AhDHN expression was suppressed. However, AhDHN2 was inhibited by sodium tungstate at 2 h, though other AhDHNs were not. AhDHNs expressions increased greatly in peanut leaves treated with 30% polyethylene glycol (PEG). Sodium tungstate along with PEG inhibited the expression of AhDHNs. This study found that exogenous and endogenous ABA can both affect the expression of AhDHN independently. The differential expression of AhDHNs to exogenous ABA may be because of differences in the structure of different AhDHNs.

Item Type: Article
Additional Information: We thank Professor Xuanqiang Liang for peanut ESTs library and seeds. This work was supported by Guangdong Natural Science Foundation (10251063101000010) and Technology Planning Project of Guangzhou City, China.
Uncontrolled Keywords: Arachis hypogaea L. dehydrins (AhDHNs), peanut, abscisic acid (ABA), expression, sodium tungstate, water stress.
Author Affiliation: Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, College of Life Science, South China Normal University, Guangzhou, 510631, P.R. China
Subjects: Crop Improvement
Divisions: Groundnut
Depositing User: Mr Siva Shankar
Date Deposited: 03 Jul 2012 07:58
Last Modified: 03 Jul 2012 07:59
Official URL: http://dx.doi.org/10.5897/AJB11.3844
URI: http://eprints.icrisat.ac.in/id/eprint/6487

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