Blair, M. W. and Izquierdo, P. (2012) Use of the advanced backcross-QTL method to transfer seed mineral accumulation nutrition traits from wild to Andean cultivated common beans. Theoretical Applied Genetics. 17 p..
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Abstract
Iron deficiency anemia and zinc deficiency are major health concerns across the world and can be addressed by biofortification breeding of higher mineral concentration in staple crops, such as common bean. Wild common beans have for the most part had higher average seed mineral concentration than cultivars of this species but have small un-commercial seeds. A logical approach for the transfer of the seed mineral trait from wild beans to cultivated beans is through the advanced backcross breeding approach. The goal of this study was to analyze a population of 138 BC2F3:5 introgression lines derived from the very high iron wild genotype G10022 backcrossed into the genetic background of the commercial-type variety ‘Cerinza’, a large-red seeded bush bean cultivar of the Andean genepool. In addition to measuring seed mineral accumulation traits and the quantitative trait loci (QTL) controlling these traits we were interested in simultaneously testing the adaptation of the introgression lines in two replicated yield trials. We found the cross to have high polymorphism and constructed an anchored microsatellite map for the population that was 1,554-cM long and covered all 11 linkage groups of the common bean genome.
| Item Type: | Article |
|---|---|
| Additional Information: | We are grateful to Alcides Hincapie for hybridizations between wild and cultivated parents, to Agobardo Hoyos for help with data analysis, to Yercil Viera for field trial management and to C. Astudillo for laboratory training. We are also grateful to Octavio Mosquera and Teresa Fowles for assistance with AAS and ICP analyses, respectively. |
| Author Affiliation: | Universidad Nacional de Colombia, Km 12 via Chapinero, Palmira, Valle, Colombia |
| Subjects: | Crop Improvement |
| Divisions: | Other Crops |
| Depositing User: | Mr Siva Shankar |
| Date Deposited: | 02 Jul 2012 06:30 |
| Last Modified: | 02 Jul 2012 06:31 |
| Official URL: | http://dx.doi.org/10.1007/s00122-012-1891-x |
| URI: | http://eprints.icrisat.ac.in/id/eprint/6422 |
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