Rosales, A. and Galicia, L. and Oviedo, E. and et al, . (2011) Near-Infrared Reflectance Spectroscopy (NIRS) for Protein, Tryptophan, and Lysine Evaluation in Quality Protein Maize (QPM) Breeding Programs. Journal of Agriculture and Food Chemistry, 59 (20). pp. 10781-10786.
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Abstract
Quality protein maize (QPM) has approximately twice the tryptophan (Trp) and lysine (Lys) concentrations in protein compared to normal maize. Because several genetic systems control the protein quality of QPM, it is essential to regularly monitor Trp and/or Lys in breeding programs. Our objective was to examine the potential of near-infrared reflectance spectroscopy (NIRS) to enhance the efficiency of QPM research efforts by partially replacing more expensive and time-consuming wet chemistry analysis. More than 276 maize samples were used to develop NIRS models for protein content (PC), Trp, and Lys. The standard error of prediction (SEP) for the calibration and the coefficient of determination for validation (R2 v) were 0.26 and 0.96 for PC, 0.005 and 0.85 for Trp, and 0.02 and 0.75 for Lys. When the NIRS models were used to evaluate 266 S2 lines from five QPM breeding populations, the coefficients of determination between NIRS and the chemical data were 0.94, 0.76, and 0.80 for PC, Trp, and Lys, respectively. Therefore, the NIRS models can be used to support the QPM breeding efforts.
| Item Type: | Article |
|---|---|
| Additional Information: | The authors thank Armando Conca and personnel from the Laboratory of Nutritional Quality of Maize at CIMMYT for their technical assistance. We also thank Gary Atlin, Twumasi Afriyie, and Bindiganavile Vivek for providing the maize samples for this work. We thank Kevin Pixley, Alan Krivanek, Axel Tiessen, and Jose Luis Araus for critical comments on the manuscript and Laura Yates for editorial assistance. |
| Uncontrolled Keywords: | NIRS, Zea mays, quality protein maize, tryptophan, lysine |
| Author Affiliation: | Global Maize Program, International Maize and Wheat Improvement Center (CIMMYT), Texcoco, Mexico |
| Subjects: | Statistics and Experimentation > Experimentation Crop Improvement > Plant Breeding |
| Divisions: | Maize |
| Depositing User: | Mr Siva Shankar |
| Date Deposited: | 02 Jul 2012 03:15 |
| Last Modified: | 02 Jul 2012 03:16 |
| Official URL: | http://dx.doi.org/10.1021/jf201468x |
| URI: | http://eprints.icrisat.ac.in/id/eprint/6395 |
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