Wong, K. C. and Watanabe, M. and Hinata, K. (1994) Fluorescence and scanning electron microscopic study on self-incompatibility in distylous Averrhoa carambola L. Sexual Plant Reproduction, 7 (2). pp. 116-121.
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Abstract
Various combinations of intermorph, selfing and intramorph pollinations were carried out in Averrhoa carambola and the pollinated pistils were observed under fluorescence and scanning electron microscopes in time-course experiments. In both compatible and incompatible pollinations, similar behavior of pollen germination and penetration was observed in the first 4 h after pollination. In compatible intermorph pollination, pollen tubes were found at the base of the transmitting tract of the style at 8 h and 24 h after pollination in both the pin and thrum morphs. With thrum flowers, selfing resulted in pollen tubes being uniformly arrested at the junction between the stigmatic and stylar tissues. Penetration of pollen tubes into the upper portion of style was observed in thrum intramorph pollination and, when the second member was treated as the gynoecial tissue under the same pollination, penetration of tubes was further enhanced. Pin flowers, on selfing, resulted in pollen tube penetration farther down the style than was the case with thrum selfing. Intramorph pollination of pin morph behaved in a similar manner to selfing and was not affected by genotypes. Beside the stamen-style dimorphism, the receptive surface of the cob stigma was larger in pin than thrum flowers. While pin pollen was round, thrum pollen was oblong in shape with pin to thrum ratio on the polar axis being 1.2 and on the equatorial axis 0.8. The stigma of pin morph belonged to the dry type, while that of the thrum resembled the wet type.
| Item Type: | Article |
|---|---|
| Additional Information: | This study was conducted while the senior author was on sabbatical leaves at the Laboratory of Plant Breeding, Faculty of Agriculture, Tohoku University, Sendai, Japan, and he acknowledges the assistance of the staff and the use of the facilities. During the period of study, he held a fellowship of the Japan Society for Promotion of Science, which he gratefully acknowledges. Last but not least, he is grateful to the University of Agriculture Malaysia for the granting of sabbatical leaves to enable him to pursue this study. |
| Uncontrolled Keywords: | Averrhoa carambola, Self-incompatibility Heterostyly, Fluorescence microscopy, Scanning electron microscopy, Pollen tube, Polymorphism |
| Author Affiliation: | Laboratory of Plant Breeding, Faculty of Agriculture, Tohoku University, Tsutsumidori Amamiyamachi 1-1, Aoba-ku, Sendai 981, Japan |
| Subjects: | Statistics and Experimentation > Experimentation Crop Improvement > Biotechnology |
| Divisions: | UNSPECIFIED |
| Depositing User: | Mr Siva Shankar |
| Date Deposited: | 29 Jun 2012 05:53 |
| Last Modified: | 29 Jun 2012 05:57 |
| Official URL: | http://dx.doi.org/10.1007/BF00230580 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/6357 |
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