Isolation and characterization of a stress responsive small GTP-binding protein AhRabG3b in peanut (Arachis hypogaea L.)

Sui, J-M and Li, R. and Fan, Q-C and et al, . (2012) Isolation and characterization of a stress responsive small GTP-binding protein AhRabG3b in peanut (Arachis hypogaea L.). Euphytica. 12 p..

[img] PDF (Online First) - Published Version
Restricted to ICRISAT researchers only

Abstract

Rab7 is a member of small GTP-binding protein family involved in intracellular vesicle trafficking from late endosome to vacuole, some of them are found to be responsive by different environmental stresses. Here we report the isolation of a Rab7 (RabG3b) cDNA from peanut (Arachis hypogaea). Overexpression of the open reading frame (ORF) of Rab7 cDNA encoding 205 amino acids in E. coli conferred the cells tolerance to various abiotic stresses. Transcription of AhRabG3b was differentially up-regulated by different environmental stimuli such as cold, dehydration, NaCl and the plant hormone ABA. Overexpression of the AhRabG3b exhibited enhanced tolerance to drought and salinity stresses in transgenic peanut. These results suggested that the AhRabG3b be a potential candidate gene responsible for both salinity and dehydration tolerance in cultivated peanut.

Item Type: Article
Additional Information: We thank Professor Chunhai Dong for assistance in English language editing. This study was financially supported by the National Natural Science Foundation of P.R. China (30871544) and the Youth Foundation of Natural Science Foundation of Shandong province (ZR2011CQ026).
Uncontrolled Keywords: Peanut (Arachis hypogaea L.), AhRabG3b, Abiotic stress, Overexpression
Author Affiliation: College of Life Science, Qingdao Agricultural University/Key Lab of Plant Biotechnology in Universities of Shandong, Qingdao 266109, China
Subjects: Crop Improvement
Divisions: Groundnut
Depositing User: Mr Siva Shankar
Date Deposited: 27 Jun 2012 08:18
Last Modified: 27 Jun 2012 08:19
Official URL: http://dx.doi.org/10.1007/s10681-012-0739-4
URI: http://eprints.icrisat.ac.in/id/eprint/6282

Actions (login required)

View Item View Item
EPrints Logo
eprints is powered by EPrints 3 which is developed by the School of Electronics and Computer Science at the University of Southampton. More information and software credits.