The use of stomatal chloroplast number for rapid determination of ploidy level in maize

Ho, I. and Wan, Y. and Widholm, J.M. and Rayburn, A.L. (1990) The use of stomatal chloroplast number for rapid determination of ploidy level in maize. Plant Breeding, 105 (3). pp. 203-210.

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Abstract

The value of using the chloroplast number in epidermal guard cells as an indirect ploidy indicator was evaluated for seed-grown and tissue culture-derived maize plants. For seed-grown plants, 2 genotypes (B89 and R75) which had both diploid and tetraploid seeds available were used; ploidy levels were determined by low cytometric analysis of nuclear suspensions from these plants. For regenerated plants, haploid and diploid levels were examined and the ploidy levels of the plants were determined by chromosome counts of cells from root tips. A positive relationship between the chloroplast number and ploidy level was observed for both seed-grown and regenerated plants. Stomatal guard cells of tetraploid plants had nearly double the number of chloroplasts as those from diploid plants. Similar results were obtained with the regenerated plants. Differences in mean chloroplast number between diploid and tetraploid seed-grown plants and between haploid and diploid regenerated plants were highly significant. The results demonstrated that counting chloroplasts in guard cells is an efficient means of screening a large number of plants for ploidy levels. In addition, it is suggested that this method can be used to detect contaminated seed lots by different ploidy seed and to distinguish plants of different genotypes

Item Type: Article
Author Affiliation: Department of Agronomy, University of Illinois, 1102 S. Goodwin, Urbana, IL 61801, USA
Subjects: Plant Production
Divisions: Maize
Depositing User: Ms K Syamalamba
Date Deposited: 22 Jun 2012 10:21
Last Modified: 22 Jun 2012 10:21
Official URL: http://dx.doi.org/10.1111/j.1439-0523.1990.tb01197...
URI: http://eprints.icrisat.ac.in/id/eprint/6209

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