Mogensen, H. L. and Ladyman, J. A. R. (1989) A structural study on the mode of action of CHAtm Chemical Hybridizing Agent in wheat. Sexual Plant Reproduction, 2. pp. 172-183.
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Abstract
This study has compared mature pollen grains while still in the anther, as well as postpollination responses, from untreated and CHA TM Chemical Hybridizing Agent-treated wheat plants using light (bright-field, phase-contrast, and fluorescence), scanning, and transmission electron microscopy. The chemical, azetidine-3-carboxylic acid (A3C), was applied at three treatment levels of 0.75, 1.0 and 1.5 kg/ha for the mature pollen investigations. It was found that the major effect of A3C on mature pollen is an alteration of the wall precursor vesicles (wp-vesicles), which form a high proportion of the contents of the mature grass pollen grain. The degree of deformation of the wp-vesicles is dose dependent. There is some evidence that increased aggregations of ribosomes are formed in treated pollen cytoplasm. Pollination studies (all at a treatment level of 1.0 kg/ha) show that, in most cases, the treated pollen does not germinate, and a high percentage of the pollen grains burst (60% burst grains in treated material compared to 28% in controls). In about 20% of the cases from treated plants, a short pollen tube forms, but no tubes were seen to grow far enough to enter the stigma hairs of the pistil.
| Item Type: | Article |
|---|---|
| Additional Information: | We are grateful to K.E. Soares and E.E. Elkema for caring for the plants and giving excellent technical assistance, to V.T. Wagner for the scanning electron microscope work and invaluable assistance with tissue processing, thick sectioning and many other phases of this study, to M.L. Rusche for assistance in tissue processing, and to W.J. Creedon for darkroom work. Support for this work by Shell Agricultural Chemical Company is gratefully acknowledged. |
| Uncontrolled Keywords: | Azetidine-3-carboxylic acid, Male sterility, Plant breeding. |
| Author Affiliation: | Department of Biological Sciences, P.O. Box 5640, Northern Arizona University, Flagstaff, AZ 86011, USA |
| Subjects: | Crop Improvement |
| Divisions: | Other Crops |
| Depositing User: | Mr Siva Shankar |
| Date Deposited: | 21 Jun 2012 10:16 |
| Last Modified: | 21 Jun 2012 10:16 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/6156 |
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