Estimation of Sucrose, Ratiinose and Stachyose in Soybean Seeds

Kennedy, I. R. and Mwandemele, O. D. and McWhirter, K. S. (1985) Estimation of Sucrose, Ratiinose and Stachyose in Soybean Seeds. Food Chemistry, 17 (2). pp. 85-93.

[img] PDF - Published Version
Restricted to ICRISAT researchers only

Abstract

Oligosaccharides have been analysed in extracts of soybean seed meal by HPLC using the Waters Dextropak C,a reversed-phase column. A procedure employing aqueous extraction of meal, and immediate analysis of extracts without further treatment except for ultrafiltration with a 25000 molecular weight cut-offfilter has been found much more convenient than gas-liquid chromatography of derivatized sugars. This procedure is recommended as the method of choice for such analyses with soybean. Hydrolysis of oligosaccharides in some extracts was observed, a process that may have significance for the use of soybeans as human food.

Item Type: Article
Additional Information: We are grateful to Mr W. Roy Day of Waters Associates for advice, the Rural Credits Development Fund of the Reserve Bank for research grants and Professor J. B. Pridham for suggestions on the manuscript.
Author Affiliation: Department of Agricultural Chemistry, t Department of Agricultural Genetics, University of Sydney, New South Wales, 2006, Australia
Subjects: Crop Improvement
Divisions: Soyabean
Depositing User: Mr Siva Shankar
Date Deposited: 21 Jun 2012 05:19
Last Modified: 21 Jun 2012 05:28
Official URL: http://dx.doi.org/10.1016/0308-8146(85)90077-9
URI: http://eprints.icrisat.ac.in/id/eprint/6142

Actions (login required)

View Item View Item