Chloroplast DNA variation in pearl millet and related species

Clegg, M.T. and Rawson, J.R.Y. and Thomas, K. (1984) Chloroplast DNA variation in pearl millet and related species. Genetics, 106 (3). pp. 449-461.

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Abstract

Using DNA fragments as hybridization probes, three different regions of chloroplast DNA (ctDNA), comprising about 25% of the total ctDNA, were investigated in Pennisetum americanum, P. flaccidum, P. orientale, P. purpureum, P. setaceum and Cenchrus setigerus. The first region included a 12 kbp EcoRI fragment containing the 23S, 16S and 5S rRNA genes, which is part of a larger duplicated region of reverse orientation. The second region was contained in a 21 kbp SalI fragment, which spans the short single-copy sequence separating the two reverse repeat structures and which overlaps the duplicated copies of the 12 kpb EcoRI fragment. The third region was a 6 kbp EcoRI fragment located in the large single-copy region of the ctDNA. A survey of 12 geographically diverse collections of P. americanum showed no indication of ctDNA sequence polymorphism, despite moderate levels of nuclear-encoded enzyme polymorphism. Interspecific and intergeneric differences were found for restriction endonuclease sites in both the small and the large single-copy regions of the ctDNA. The reverse repeat structure showed identical restriction site distributions in all materials surveyed. The results suggest that the reverse repeat region is differentially conserved during the evolution of the chloroplast genome

Item Type:	Article
Author Affiliation:	Botany Department, Georgia University, Athens, Georgia 30602, USA
Subjects:	Crop Improvement
Divisions:	Millet
Depositing User:	Ms K Syamalamba
Date Deposited:	29 May 2012 10:24
Last Modified:	29 May 2012 10:24
Official URL:	http://www.ncbi.nlm.nih.gov/pmc/articles/PMC122424...
URI:	http://eprints.icrisat.ac.in/id/eprint/5903

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