Rosen, H. (1957) A Modified Ninhydrin Colorimetric Analysis for Amino Acids. Archives of Biochemistry and Biophysisc, 67. pp. 10-15.
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Abstract
In 1948, Stein and Moore published the first practical procedure for the chromatographic separation of amino acids on starch columns, and a photometric method for the quantitative analysis of the effluent fractions (1, 2). Their procedure was based on the reaction of amino acids with reduced ninhydrin (triketohydrindenhydrate) at pH 5; stannous chloride served as the reducing agent. The method is reliable and reproducible.
| Item Type: | Article |
|---|---|
| Additional Information: | The work of Dr. Sterling Chaykin, who correlated much of the experimental data, is much appreciated. The advice and interest of Dr. Stanley M. Levenson are gratefully acknowledged. |
| Author Affiliation: | Department of Surgical Metabolism, Division of Surgery, Walter Reed Army Institute of Research, Walter Reed Army Medical Center, Washington, D. C. |
| Subjects: | Plant Physiology and Biochemistry > Biochemistry |
| Divisions: | General |
| Depositing User: | Mr Siva Shankar |
| Date Deposited: | 29 May 2012 04:04 |
| Last Modified: | 29 May 2012 04:04 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/5864 |
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