Schmidt-Heydt, M. and Geisen, R. (2007) A microarray for monitoring the production of mycotoxins in food. International Journal of Food Microbiology, 117. pp. 131-140.
![[img]](http://eprints.icrisat.ac.in/style/images/fileicons/application_pdf.png) |
PDF
- Published Version
Restricted to ICRISAT researchers only |
Abstract
A microarray, which covers most of the known relevant mycotoxin biosynthesis genes, has been developed. The microarray carries oligonucleotides of the fumonisin, the aflatoxin, the ochratoxin, the trichothecene (type A and B) and the patulin biosynthesis pathways. For trichothecene producing Fusaria the biosynthesis cluster of trichothecene producing Fusarium sporotrichioides (type A) and of Gibberrella zeae (type B, teleomorph of F. graminearum) have been spotted. The aflatoxin cluster carries oligonucleotides specific for Aspergillus flavus. The ochratoxin pattern is specific for ochratoxin A producing Penicillia, the fumonisin cluster is specific for G. moniliformis (teleomorph of F. verticillioides) and the patulin genes have been obtained from Penicillium expansum. The microarray is designed in a way that newly identified pathway genes can be added easily at any time. The microarray was used to detect the activation of all gene clusters under conditions conducive for mycotoxin biosynthesis. According to the results the obtained signals were specific under the hybridization conditions used and only insignificant cross-hybridizations occurred. The microarray was used to demonstrate differences in mycotoxin pathway gene expressions after growth on various media for trichothecene and ochratoxin A biosynthesis. It was used further to study and compare the expression kinetics of the trichothecene biosynthesis genes
| Item Type: | Article |
|---|---|
| Additional Information: | This work was supported by the Landesstiftung Baden- Württemberg (trichothecene gene expression) and by the EUproject “Development of cost-effective control and prevention strategies for emerging and future foodborne pathogenic microorganisms throughout the food chain” (PathogenCombat), FOOD-CT-2005-07081 (ochratoxin gene identification and gene expression). We would like to thank Nicole Mischke, Karla Hell and Lars Uhlmann for skilful technical assistance. We also would like to thank Dr. Sandra Masloff and Dr. W. Herman for the analysed wheat samples. |
| Uncontrolled Keywords: | Microarray; Gene expression; Trichothecene; Aflatoxin; Ochratoxin; Fumonisin |
| Author Affiliation: | Federal Research Centre for Nutrition and Food, Location Karlsruhe, Haid-und-Neu-Str. 9 76131 Karlsruhe, Germany |
| Subjects: | Plant Protection |
| Divisions: | UNSPECIFIED |
| Depositing User: | Mr Siva Shankar |
| Date Deposited: | 23 May 2012 07:57 |
| Last Modified: | 23 May 2012 07:57 |
| Official URL: | http://dx.doi.org/10.1016/j.ijfoodmicro.2007.01.01... |
| URI: | http://eprints.icrisat.ac.in/id/eprint/5655 |
Actions (login required)
 |
View Item |
