Price, M. S. and Conners, S. B. and Tachdjian, S. and et al, . (2005) Aflatoxin conducive and non-conducive growth conditions reveal new gene associations with aflatoxin production. Fungal Genetics and Biology, 42. pp. 506-518.
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Abstract
Research on aXatoxin (AF) production has traditionally focused on deWning the AF biosynthetic pathway with the goal of identifying potential targets for intervention. To understand the eVect of nitrogen source, carbon source, temperature, and pH on the regulation of AF biosynthesis, a targeted cDNA microarray consisting of genes associated with AF production over time was employed. Expression proWles for genes involved in AF biosynthesis grouped into Wve clades. A putative regulon was identiWed consisting of 20 genes that were induced in the conducive nitrogen and pH treatments and the non-conducive carbon and temperature treatments, as well as four other putative regulons corresponding to each of the four variables studied. Seventeen genes exhibited consistent induction/ repression proWles across all the experiments. One of these genes was consistently downregulated with AF production. Overexpression of this gene resulted in repression of AF biosynthesis. The cellular function of this gene is currently unresolved.
| Item Type: | Article |
|---|---|
| Additional Information: | The authors thank Kim Schwartzburg, Carrie Jacobus, Rob Holmes, and Greg OBrian for their critique of the manuscript. The authors would additionally like to thank Matt Johnson and Clemente Montero for their assistance in the construction and hybridization of the microarrays. This research was supported by Grant 99- 35201-8073 from the USDA/NRI Competitive Grants Program. |
| Uncontrolled Keywords: | AXatoxin; Gene expression; Regulation; Aspergillus Xavus; Aspergillus parasiticus |
| Author Affiliation: | Center for Integrated Fungal Research, North Carolina State University, Raleigh, NC 27695-7244, USA |
| Subjects: | Crop Improvement |
| Divisions: | General |
| Depositing User: | Mr Siva Shankar |
| Date Deposited: | 23 May 2012 07:51 |
| Last Modified: | 23 May 2012 07:51 |
| Official URL: | http://dx.doi.org/10.1016/j.fgb.2005.03.009 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/5653 |
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