Pedersen, W.L. and Chakrabarty, K. and Klucas, R.V. and Vidaver, A.K. (1978) Nitrogen fixation (acetylene reduction) associated with roots of winter wheat and sorghum in Nebraska. Applied and Environmental Microbiology, 35 (1). pp. 129-135.
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Abstract
Root segments and root-soil cores (6.5-cm diameter) from fields and nurseries of winter wheat and sorghum were tested for N2 fixation by acetylene reduction assay. Wheat samples (about 1,200) from 109 sites generally had low or no activity (0 to 3.1 nmol of C2H4/ha/g [dry weight] of root segments), even after 24 h of incubation. However, a commercial field of Scout 66 in western Nebraska, exhibited appreciable activity (290 nmol of C2H4/ha/g [dry weight] of root segments). Of 400 sorghum lines and crosses, grain sorghums (i.e., CK-60A, Wheatland A, B517, and NP-16) generally exhibited higher nitrogenase activity than did forage sorghums or winter wheats. CK-60A, a male sterile grain sorghum sampled at four locations, had the most consistent activity of 24 to 1,100 nmol of C2H4/ha/core. The maximum rate extrapolated to 2.5 g of N/ha/day. Numerous N2-fixing bacterial isolates were obtained from wheat and sorghum roots that exhibited high nitrogenase activity. Most isolates were members of the Enterobacteriaceae, i.e., Klebsiella pneumoniae, Enterobacter cloacae, and Erwinia herbicola
| Item Type: | Article |
|---|---|
| Author Affiliation: | Laboratory of Agricultural Biochemistry, University of Nebraska, Lincoln, Nebraska 68583, USA |
| Subjects: | Plant Production Plant Physiology and Biochemistry > Plant Physiology Plant Physiology and Biochemistry > Biochemistry |
| Divisions: | Sorghum |
| Depositing User: | Ms K Syamalamba |
| Date Deposited: | 22 May 2012 08:56 |
| Last Modified: | 22 May 2012 08:59 |
| Official URL: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC242791... |
| URI: | http://eprints.icrisat.ac.in/id/eprint/5612 |
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