Maden, S. and Singh, D. and Mathur, S. and Neergaard, P. (1975) Detection and location of seed-borne inoculum of Ascochyta rabiei and its transmission in chickpea (Cicer arietinum). Seed Science and Technology, 3 (3-4). pp. 667-681.
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Abstract
A. rabiei infected 70% of C. arietinum samples from C. Anatolia, Turkey. The standard blotter method, using 5 seeds/Petri dish and 12 h NUV 12 h darkness cycles at 22 deg C, proved most suitable for detecting the fungus. The inoculum was spore contamination and mycelium occurred in the seed coat alone or in seed coat and embryo. Pycnidia were observed only in the seed coat of seeds having deep lesions. Inter- and intracellular mycelium was localized to lesions. Pycnidia were subepidermal and contained mature spores. Pycnidiospores obtained from the seed surface and pycnidia from 14-month-old seed stored at 3 plus or minus 2 deg showed 33% germination. The light and temp. responses of the fungus on PDA revealed that opt. colony growth occurred at 19 deg . Pycnidial formation was max. under NUV but failed in darkness. Both superficial and deep infections were equally potent in the transmission of the disease. The fungus was highly pathogenic to seed and 40-day-old plants.This paper was presented at the 17th Int. Seed Testing Congress 1974.
| Item Type: | Article |
|---|---|
| Author Affiliation: | Danish Gov. Institute Seed Path. Developing Countries, Hellerup, Copenhagen, Denmark. |
| Subjects: | Plant Protection Crop Improvement |
| Divisions: | Chickpea |
| Depositing User: | Mr. SanatKumar Behera |
| Date Deposited: | 21 May 2012 13:23 |
| Last Modified: | 21 May 2012 13:24 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/5586 |
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