Tajima, K. and Shimizu, N.
(1977)
Effect of membrane stabilizers and polyhydric alcohols on chilling injury of sorghum seedlings.
Japanese Journal of Crop Science, 46 (3).
pp. 335-342.
Abstract
30 seedlings of sorghum cv. Common or Sweet were grown in each pot (150 X 40 X 100 mm) containing volcanic ash soil given 0.1 g N, P and K, and were grown under day/night conditions of 30/20 deg C, at RH >90%, until the 3rd leaf was fully expanded. Each pot was given 200 ml aqueous sol. of (a) acetylsalicyclic acid methyl ester, (b) ethoxybenzamide, (c) isobutylphenylacetic acid, (d) diphenhydramine hydrochloride, (e) ethyl p-aminobenzoate, (f) IAA, (g) NAA, (h) 4-chloro-2-methylphenoxyacetic acid, (i) theophylline, (j) theophylline + IAA, (k) sodium beta -glycerophosphate, (l) dihydroxyacetone (dimer), (m) xylitol, (n) mannitol, (o) inositol, (p) glyoxal, (q) glutaraldehyde, (r) p-phthalaldehyde, (s) diacetyl, (t) acetylacetone, (u) acetonylacetone or was given 200 ml water (control), and left overnight. Seedlings for experiments with (f), (g) and (h) were grown in vermiculite, and watered with a sol. containing NH4NO3, KH2PO4, KCl and ferric citrate. The seedlings were subjected to chilling at 6 deg for 5-7 days in the dark, and subsequently transferred to 15 deg for 1-3 days under fluorescent light, 20 deg for 3 days in daylight and finally to 27 deg . The leaves of untreated seedlings showed severe wilting when transferred to 15 deg , resulting in leaf injury and frequently plant death on transfer to 20 and 27 deg . Chilling injury was reduced by (a), (b), (c), (d) and (e), which have a similar effect to cholesterol on membrane stabilization and with the exceptions of (a) at 2.6 X 10-2 M, (d) at 1 X 10-2 M and (e) percentage seedling survival at 27 deg was increased. A decrease in leaf wilting was found with (f) at 10-4-10-5 M, (g) and (h) at 10-5-10-6 M, (i) at 5.6 X 10-3 M and (j); increased survival was observed with these treatments, except (h) at 10-5 M. The glycerol derivatives and polyhydric alcohols (h), (l), (m), (n) and (o) decreased leaf wilting and increased survival; these inhibit low temp. denaturation of proteins. Growth of seedlings was adversely affected by (q), but (p), (r), (t) and (u) gave decrease in leaf wilting and increased seedling survival, and (s) at 1.2 X 10-2 M gave a significant reduction in leaf wilting
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