Taylor, J.R.N. and Schussler, L. (1984) Sorghum cultivar verification by electrophoresis. Journal of the Science of Food and Agriculture, 35 (1). pp. 1-8.
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Abstract
After comparing the merits of three different electrophoretic methods of separating cereal prolamins, a procedure for sorghum cultivar verification by urea-polyacrylamide gel electrophoresis has been developed. The problem of the tannins in high-tannin (bird-resistant) cultivars rendering the prolamins insoluble has been overcome by extracting the proteins in the presence of polyvinylpolypyrrolidone. As with other cereals, the electrophoretic pattern of the prolamins was unaffected by the environment in which the plants were grown. Further, the pattern was not affected by long-term storage of the grain, and cultivars appear to remain unique with respect to their prolamin proteins through many generations
| Item Type: | Article |
|---|---|
| Author Affiliation: | Sorghum Beer Unit, Council for Scientific and Industrial Research, P.O. Box 395, Pretoria, 0001, South Africa |
| Subjects: | Plant Production |
| Divisions: | Sorghum |
| Depositing User: | Ms K Syamalamba |
| Date Deposited: | 14 May 2012 07:52 |
| Last Modified: | 14 May 2012 07:52 |
| Official URL: | http://onlinelibrary.wiley.com/doi/10.1002/jsfa.27... |
| URI: | http://eprints.icrisat.ac.in/id/eprint/5299 |
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